Altered ERK 1/2 and Smad 1/5/8 phosphorylation observed in primary rat hippocampal cells upon iAβ expression.

<p><b>(A)</b> Immunoblot of phospho-ERK 1/2, total-ERK 1/2, and MYC-Tag in VC or iAβ transduced HPC neurons following BDNF stimulation. Myc-tag observed in lanes corresponding to iAβ transduced hippocampal cells and absent in VC samples. Decreased ERK 1/2 phosphorylation in iAβ expressing cells is rescued upon pre-treatment with PLX4032. Two-dimensional gel transformation of phospho-ERK 1/2 intensities performed and shown as histogram. Beta-actin used as loading control. <b>(B)</b> Plot of relative densitometry analysis of phospho-ERK 1/2 with data corresponding to biological replicates shown as mean ± SE (n = 3). Statistically significant (p < 0.05) decrease in phospho-ERK 1/2 observed in iAβ transduced cells restored upon pre-treatment with PLX4032. Complete pairwise analysis shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191696#pone.0191696.t005" target="_blank">Table 5</a>. <b>(C)</b> Immunoblot of phospho-Smad 1/5/8 and total-Smad 1/5/8 in VC or iAβ transduced HPC neurons following rh-BMP2 stimulation. <b>(D)</b> Plot of densitometry analysis of biological replicates shown as a mean ± SE (n = 3) depicting an increase in sensitivity, approaching statistical significance (p < 0.0517), of iAβ transduced hippocampal cells relative to VC. Complete pairwise analysis shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191696#pone.0191696.t006" target="_blank">Table 6</a>.