PKC mediated activation of MAPK and AKT pathways by KA stimulation.

<p>(A) Western blot analysis of time-dependent activation of JAK1, STAT1, AKT, MAPK ERK1/2 and p38 after adding 100μM KA to 8 div hippocampal neurons for 30min. (B) Pretreatment of 8 div hippocampal neurons with PKC inhibitor (Staurosporine, 0.1μM) compromised the KA-induced activation of AKT, MAPK ERK1/2 and p38, but had no effect on the activation of JAK1 and STAT1. (C) Pretreatment of 8 div hippocampal neurons with PKA inhibitor (H89, 40μM) had no effect on the activation of JAK1, STAT1, AKT, MAPK ERK1/2 and p38 induced by KA. (D) Pretreatment of primary hippocampal neurons with MAPK-p38 inhibitor (Skepinone-L, 20μM) resulted in inhibition of KA-induced expression of MHC-I. (E) Pretreatment of primary hippocampal neurons with MAPK-ERK inhibitor (U0126, 10μM) blocked KA-induced expression of MHC-I. (F) Pretreatment of primary hippocampal neurons with MAPK-p38 inhibitor (Skepinone-L, 20μM) blocked KA-induced expression of p-p65, p-CREB and IRF-1. (G) Pretreatment of primary hippocampal neurons with MAPK-ERK inhibitor (U0126, 10μM) resulted in inhibition of KA-induced expression of IRF-1 and activation of NF-κB p65, but had no effect on the activation of CREB.