TRAF1 is necessary for oligo-HA to suppress IL-6 secretion and mRNA expression in peritoneal macrophages.

<p>Peritoneal macrophages from WT (A, C) and <i>Traf1−/−</i> mice (B, D) were treated with oligo-HA and/or poly(I:C) for 24 h. IL-6 release in cultured media was measured by ELISA (A, B). mRNA abundance for IL-6 was assessed by quantitative RT-PCR 4 h after treatment (C, D). Quantitative RT-PCR data are presented as normalized data against vehicle-treated macrophages. Data are presented as means ± S.E.M. and are representative of three independent experiments with n = 3. **: p<0.01. ***: p<0.001.