Oligo-HA suppresses poly(I:C) induced, but not LPS-induced cytokine release in MH-S cells.

<p>MH-S cells were treated with vehicle (PBS), oligo-HA (10 µg/ml), oligo-HA+poly(I:C) (10 µg/ml), or poly(I:C) alone for 24 h. IL-6 (A) and TNFα (B) release in the cultured media was measured by ELISA. mRNA abundance for IL-6 (C) and TNFα (D) was assessed 4 h after treatment by quantitative RT-PCR. Quantitative RT-PCR data are shown as relative expression as compared with vehicle-treated macrophages. (E) MH-S cells were treated with 100 ng/ml LPS in the presence or absence of oligo-HA for 24 hours. IL-6 release in the cultured media was measured by ELISA. Data are representative of five independent experiments with n = 3 per group and are means ± S.E.M. ***: p<0.001. **: p<0.01.