Gating strategies for CMV-SP-CD4 T cells.

<p>All flow cytometry plots are from a single subject of the RESTORE study. Lymphocytes were identified using forward and side scatter (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077479#pone-0077479-g001" target="_blank">Figure 1A</a>), followed by gating on CD4+ T cells (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077479#pone-0077479-g001" target="_blank">Figure 1B</a>). Gates for CD25+ and CD134+ cells were placed based on comparison with negative control (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077479#pone-0077479-g001" target="_blank">Figure 1C</a>) and PHA positive control (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077479#pone-0077479-g001" target="_blank">Figure 1D</a>) to include cells highly co-expressing CD25 and CD134. A representative example of the dynamics of responses to CMV antigens over 96 weeks of follow-up in a RESTORE subject (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077479#pone-0077479-g001" target="_blank">Figure 1E-K</a>).