A) Singlets, B) lymphocytes, C) T-lymphocytes, D) CD4 and CD8 T-lymphocytes, E) Effector Memory 1 CD4, F) Central Memory and Naive CD4, G) Effector Memory 2 and Effector Memory 3, H) 4th Antigen Experienced, I) Naive CD8 and 1st Antigen Experienced, J) 2nd and 3rd Antigen Experienced.</p
Gating strategy for T and B cell panels are shown. Naïve CD4+ or CD8+ T cells were defined as CD28+/...
Shown are gating strategies for the analysis of (A), human immune cell subsets (hCD45+CD3+: T cells,...
<p>a) Viable cells were gated based on negative expression of the fixable viability stain (FVS). Tru...
A) CD8 T-lymphocytes gated by CD45RA and CCR5, B) CD8 T-lymphocytes gated by FSC-H and CCR5, C) Naïv...
A) CD4 T-lymphocytes gated by CD45RA and CCR5, B) CD4 T-lymphocytes gated by FSC-H and CCR5, C) Naïv...
<p>These plots represent the gating strategy used for flowcytometry analysis of blood samples; A, ly...
<p>These plots represent the gating strategy used for flowcytometry analysis of blood samples; lymph...
<p>2×10<sup>6</sup> Fresh splenocytes were stained with PE-conjugated H-2K<sup>d</sup> AMQMLKETI MHC...
<p>At least 30,000 lymphocytes were evaluated and were gated based on their forward (FCS; X-axis) an...
<p>CD4<sup>+</sup> T cells (B) were gated from lymphocytes (A) for further phenotyping. (C, D, E) Re...
<p>The proportions of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and Tregs in PBMCs and in pan...
<p>All lymphocytes (gate O) were gated by forward scatter/side scatter (FS/SS), differentiating T ce...
<p><b>A)</b> PBMC were stimulated overnight and cells were analysed by flow cytometry for expression...
(A) Lymph node samples were gated for total lymphocytes, singles cells, and live cells. Live cells w...
<p>We identified T cells from total leukocytes as shown. We first selected singlets using forward sc...
Gating strategy for T and B cell panels are shown. Naïve CD4+ or CD8+ T cells were defined as CD28+/...
Shown are gating strategies for the analysis of (A), human immune cell subsets (hCD45+CD3+: T cells,...
<p>a) Viable cells were gated based on negative expression of the fixable viability stain (FVS). Tru...
A) CD8 T-lymphocytes gated by CD45RA and CCR5, B) CD8 T-lymphocytes gated by FSC-H and CCR5, C) Naïv...
A) CD4 T-lymphocytes gated by CD45RA and CCR5, B) CD4 T-lymphocytes gated by FSC-H and CCR5, C) Naïv...
<p>These plots represent the gating strategy used for flowcytometry analysis of blood samples; A, ly...
<p>These plots represent the gating strategy used for flowcytometry analysis of blood samples; lymph...
<p>2×10<sup>6</sup> Fresh splenocytes were stained with PE-conjugated H-2K<sup>d</sup> AMQMLKETI MHC...
<p>At least 30,000 lymphocytes were evaluated and were gated based on their forward (FCS; X-axis) an...
<p>CD4<sup>+</sup> T cells (B) were gated from lymphocytes (A) for further phenotyping. (C, D, E) Re...
<p>The proportions of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and Tregs in PBMCs and in pan...
<p>All lymphocytes (gate O) were gated by forward scatter/side scatter (FS/SS), differentiating T ce...
<p><b>A)</b> PBMC were stimulated overnight and cells were analysed by flow cytometry for expression...
(A) Lymph node samples were gated for total lymphocytes, singles cells, and live cells. Live cells w...
<p>We identified T cells from total leukocytes as shown. We first selected singlets using forward sc...
Gating strategy for T and B cell panels are shown. Naïve CD4+ or CD8+ T cells were defined as CD28+/...
Shown are gating strategies for the analysis of (A), human immune cell subsets (hCD45+CD3+: T cells,...
<p>a) Viable cells were gated based on negative expression of the fixable viability stain (FVS). Tru...