Representative plot illustrating CD25 gating strategy.
- Melinda S. Suchard (363133)
- Elizabeth Mayne (363134)
- Victoria A. Green (363135)
- Sharon Shalekoff (363136)
- Samantha L. Donninger (363137)
- Wendy S. Stevens (144114)
- Clive M. Gray (88321)
- Caroline T. Tiemessen (204853)
Publication date
February 2013
DOIAbstract
<p>Lymphocytes were gated according to forward and side scatter. CD3 positive lymphocytes were selected. CD25 expression on the x-axis was plotted against CD4 on the y-axis. A gate was set according to CD25 expression on the CD4<sup>−</sup> population. This gave a percentage of CD3<sup>+</sup>CD4<sup>+</sup>CD25<sup>+</sup> lymphocytes as a percentage of CD3<sup>+</sup> (figure in top-right quadrant). This percentage was then used to calculate the percentage of CD3<sup>+</sup>CD4<sup>+</sup>CD25<sup>+</sup> lymphocytes as a percentage of the CD3<sup>+</sup>CD4<sup>+</sup> population (CD4 population being top-left and right quadrants added together).</p
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