Flow cytometry gating strategy for T and B cell panels.

Gating strategy for T and B cell panels are shown. Naïve CD4+ or CD8+ T cells were defined as CD28+/CD95-, central memory CD4+ or CD8+ T cells were defined as CD28+/CD95+, and effector memory CD4+ or CD8+ T cells were defined as CD28-/CD95+. Naïve B cells were defined as IgD+/CD27-, MZ-like B cells were defined as IgD+/CD27+, and memory B cells were defined as IgD-/CD27+. Proliferating (Ki67+) T and B cells and granzyme B expressing (granzyme B+) T cells within each subset were also quantified using these gating schemes. (EPS)