Modification of four of the candidate miRNA expression restored Gem sensitivity to HuH28 cells.

<p>Ectopic overexpression of miR-29b (A), miR-205 (B), or miR-221 (C) via transfection of a corresponding miRNA mimic and downregulation of miR-125a-5p (D) via transfection of an anti miRNA oligonucleotide made HuH28 cells more sensitive to Gem. Relative cell viabilities were assessed 72 hr after Gem treatment. The final concentration of each miRNA mimics was 10 nM; that of the anti miRNA oligonucleotide was 40 nM. Mock: receiving only transfection reagent. siCON: control treated with a non-silencing miRNA mimic. AntiOligoCON: control treated with a non-silencing control oligonucleotide. The asterisk denotes p<0.05 as compared to non-treated control, mock and siCON or control AntiOligoCON.