miR-155 promotes cell proliferation in U251 and A172 cells.

<p>(A) U251 cells were transfected with a mimic control, a miR-155 mimic, an inhibitor control, or miR-155 inhibitor, and the growth of cell was investigated using an MTT assay after 72 h. The absorbance at 490 nm was normalized to the control to determine cell viability. The given values represent the means ± SD. (B, C) U251 cells were transfected with a mimic control, a miR-155 mimic, an inhibitor control, or a miR-155 inhibitor as indicated, and an EdU incorporation assay was carried out (scale bar, 50 µm). (D, E) A172 cells were transfected with a mimic control, a miR-155 mimic, an inhibitor control, or a miR-155 inhibitor as indicated, and an EdU incorporation assay was carried out (scale bar, 50 µm). The EdU incorporation rate was expressed as the ratio of the number of EdU-positive cells to the total number of Hoechst 33342-positive cells. The average values ± SD of three separate experiments were plotted, * <i>P</i><0.05, ** <i>P</i><0.01.