Two of software predicted miRNA target genes actually suppressed by corresponding miRNA.

<p>(A) MMP-2 and (B) PIK3R1 were significantly suppressed by transfection of the respective miRNA mimics. Selective downregulation of MMP-2 (C) or PIK3R1 (D) by transfection of each corresponding siRNA conferred Gem sensitivity to HuH28 cells. The analysis of Western blot and relative cell viability were performed 72 hours after Gem treatment. Final concentration of siRNAs and miRNA mimics were 10 nM. Mock: receiving only transfection reagent. siCON: control treated with a non-silencing miRNA mimic. The asterisk denotes p<0.05 as compared to non-treated control, mock and siCON.