Immunosuppression in the lymph nodes of B6.WT and B6.CCR7^-/- during infection with <i>L. major</i>.

<p>(A) Expression of IL-10 in the total lymph node, as well as in the CD4⁺ and CD8⁺ compartments was determined by flow cytometry in B6.WT (closed circles) and B6.CCR7^-/- (open circles) mice. (B) CD4⁺ or CD8⁺ Tregs were determined by their dual expression of CD25 and Foxp3. (C) The total percentage of Foxp3⁺ cells as well as the percentage of CD4⁺ and CD8⁺ Tregs in the total population was determined by intracellular flow cytometry. (D) Mice were injected intraperitoneally with PBS, anti-IL-10R or anti-IL-4, and the draining lymph node analysed for the percentage of CD4⁺ Tregs at day 14 post infection. A&C, One representative analysis of three experiments; Experimental group size: n=5 mice/genotype for each timepoint; D, One experimental analysis; Experimental group size: n=3 mice/genotype for each treatment. *p<0.05; **p<0.01.