IL-10 and Foxp3 expression in CD4⁺ LN T-cells of untreated or CD28SA treated C57BL/6 mice.

<p>(<b>A</b>) IL-10 and Foxp3 expression in unstimulated mice and in mice 3 days after CD28SA treatment. (<b>B</b>) Kinetics of subsets defined by CD8, CD4, Foxp3 and IL-10 expression 0 to 8 days after CD28SA injection. (<b>C</b>) Fold change in cell numbers between day 0 and day 3 after stimulation. Graphs show means ± SD from 3 mice assayed individually and results are representative of three independent experiments. (<b>D</b>) Lack of conversion of CD4⁺ Tconv after CD28SA treatment. Sorted Thy1.2⁺CD4⁺ Foxp3⁻ cells from DEREG mice were transferred into naïve Thy1.1 mice one day before CD28SA injection. Three days later Thy1.2⁺ cells were analysed for Foxp3 and IL-10 expression. Graphs show means ± SD from 4 mice assayed individually and results are representative of two independent experiments. (<b>E</b>) Proliferative history and expression of Foxp3 and IL-10. CFSE-labeled CD4⁺ T-cells from Thy1.1 mice were transferred into naïve C57BL/6 mice one day before CD28SA injection. Three days later Thy1.1⁺ cells were analysed for proliferation, Foxp3 and IL-10 expression. Flow cytometric analysis of transferred T-cells with average numbers of cell division (acd) (left) and percentage of IL-10 producers within Foxp3⁺ cells per generation (right) are depicted. Data represent pooled cells from two mice, and results are representative of three independent experiments.