Characterization of the early inflammation in <i>L. major</i>-infected T cell-specific IL-10-deficient C57BL/6 mice.

<p>The early enhanced inflammation in T cell-specific and complete IL-10-deficient C57BL/6 mice is not associated with changes in the cell numbers but correlates with elevated secretion of IFN-γ by CD4⁺ and CD8⁺ cells. C57BL/6 IL-10 mutant mice were infected with <i>L. major</i> promastigotes into the right hind footpad. Increase in size of the infected compared with the non-infected footpads (<b>A</b>). Frequencies of cell populations in the infected feet (<b>B</b>). Cell numbers in the draining lymph nodes (<b>C</b>) and frequencies of cell populations in the draining lymph nodes (<b>D</b>). The results show the mean of two independent experiments with 2 mice per group and time point (<b>A to D</b>). Secretion of IFN-γ (<b>E</b>) and IL-4 (<b>F</b>) by draining lymph node cells 7 days after infection with <i>L. major</i>. Data show the mean ± SEM of four independent experiments with 2 mice per group and experiment (<b>E and F</b>). Draining lymph node cells of infected mice were stained for IFN-γ and the indicated surface markers; the fraction of double positive cells of all lymph node cells is shown. The results are representative of two experiments (<b>G</b>).