CD4 and CD8 T cells are primary source of IL-10 during early <i>L. major</i> infection.

<p>IL-10-eGFP reporter mice (Vert-X mice) were infected intradermally in the ear <i>L. major</i> metacyclic promastigotes. Some mice were treated with anti-IL-10R mAb as described in the <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003243#s4" target="_blank">material and methods</a>. One week after infection, cells from the ear were prepared and stained for CD11b, CD19, TCRγδ, NK1.1, CD8 and CD4 T cells. Additional intracellular staining was performed for Foxp3 and IFN-γ. Frequency of IL-10-eGFP⁺ cells on different populations (A). Gated Foxp3⁺ or Foxp3⁻ CD4⁺ T cells depicting IL-10-eGFP expression (B) and IFN-γ production (C) in infected mice are shown. Number represents percentage of IL-10-eGFP on each gated population. Histogram showing the frequency (right) and number (left) of Foxp3⁺ or Foxp3⁻ CD4T cells expressing IL-10-eGFP (D). Values are mean ± SEM of 5 mice per group and representative of two experiments (*, <i>p</i><0.05).