IL-10^GFP+CD4⁺ T cells from the skin of mice infected with <i>S</i>. <i>mansoni</i> suppress proliferation of CD4⁺ T cells in the skin draining lymph nodes.

<p>(<b>A</b>) Representative flow cytometry dot plots of CFDA-SE^dim CD3⁺CD4⁺ cells from the sdLN of 1x infected mice after co-culture for 96h with dermal CD4⁺ T cells from 4x infected WT or IL-10^-/- mice in the presence of parasite antigen SSAP. (<b>B</b>) Data is given as sdLN CD3⁺CD4⁺ T cell proliferation expressed as the fold-change observed in each of seven corresponding mice co-cultured in the presence of dermal CD4⁺ T cells from either WT or IL-10^-/- mice relative to sdLN CD3⁺CD4⁺ T cells cultured without added dermal CD4⁺ T cells. Symbols are individual mice; horizontal bars are means ± SEM; n = 7. (<b>C</b>) Representative flow plots and (<b>D</b>) fold change ± SEM of CFDA-SE^dim sdLN CD3⁺CD4⁺ T cells from 1x infected mice (n = 8) co-cultured with GFP⁺, or GFP^Neg dermal CD4⁺ T cells from 4x infected mice in the presence of SSAP. Differences between the means of selected groups were compared by ANOVA and Tukey’s multiple comparisons test analysis (* = p<0.05; ** = p<0.01; *** = p<0.001; **** = p<0.0001; ns = p>0.05).