Lack of CD4⁺ T cells ablates IL-10 production induced in skin exposed to <i>S</i>. <i>mansoni</i> cercariae.

<p>(<b>A</b>) Proportions of CD11b^highGr1⁺ neutrophils and (<b>B</b>) SiglecF⁺F4/80⁺MHC-II^- eosinophils in total DEC recovered from 1x, or 4x infected WT and Rag2^-/- mice on day 4 post infection. Symbols are values for cells obtained from independent tissue samples; horizontal bars are the means ± SEM; n = 4–6 pinnae per group. Production of (<b>C</b>) IL-10 and (<b>D</b>) IL-4 in overnight culture supernatants of skin biopsies from 1x or 4x infected WT and Rag2^-/- mice obtained on day 4 post-final infection; bars are means + SEM, n = 4–6 pinnae per group, as measured by ELISA. Significant differences means were compared between of selected groups by ANOVA and post-hoc tests (Sidak’s and Tukey’s) (* = p<0.05; ** = p<0.01; *** = p<0.001; **** = p<0.0001; ns = p>0.05).