Effects of <i>vps-52</i> on the miRNA-mediated regulation of vulva and ASEL neuron development.

<p><b>A</b>) Analysis of the <i>let-60</i>-mediated Muv phenotype. Left panels: Nomarski pictures of adult hermaphrodites of the indicated genotypes. A single vulva (white arrowhead) is observed in the GARP mutant. In <i>let-60(n1046)</i> mutants multiple vulval-like structures (black arrowheads, Muv phenotype) are observed in addition to vulva (white arrowhead). Scale bar measures 100 µm. Right panel: Percentage of adult worms of the indicated genotypes with the Muv phenotype. <b>B</b>) Analysis of the <i>lsy-6</i>-mediated ASEL-specific gene expression. Left Panels: GFP fluorescence pictures of the <i>lim-6</i>p::GFP reporter. In wild type (WT) animals, gene silencing mediated by the <i>lsy-6</i> miRNA leads to GFP expression in the ASEL neuron (arrowheads). Impairment of the miRNA activity in <i>vps-52(qbc4)</i>; <i>lsy-6(ot150)</i> mutant blocks reporter expression. Scale bar measures 50 µm. L: Left, R: Right, H: Head, T: Tail. Right panel: Percentage of adult worms of the indicated genotypes that failed to express the <i>lim-6</i>p::GFP reporter in ASEL. The strains were grown and scored at 15°C. The number of animals scored (n) is indicated.