Effects of <i>vps-52</i> on the GW182 protein and GW182 mutant animals.

<p><b>A</b>) Abundance of the GW182 protein, AIN-1. (Top) Populations of the indicated genotyped were synchronized to the adult stage and Western blot performed on these samples. Actin served as loading control. (Bottom) Quantification of the AIN-1 signal compared with the level found in wild type worms (WT: 1). The error bars represent standard deviation from three independent experiments. <i>p</i> values were obtained using a two-sided Student's test. <b>B</b>) Synergy between <i>vps-52</i> and <i>ain-1</i> mutants. The indicated strains were grown at 15°C and the continuity of the cuticular alae analyzed in young adult animals. The number of animals scored (n) is indicated.