Seam cell-related phenotypes of the GARP mutants.

<p><b>A</b>) Percentage of young adult worms with defective cuticular alae. Young adult hermaphrodites from synchronized populations were mounted and analyzed under Nomarski optics. The continuity of the cuticular alae was scored and categorized as gapped (one or more interruptions) or complete (no gaps present). Strains above the horizontal black line were scored at 20°C, those below that line were grown and scored at 15°C. <b>B</b>) Average and range of seam cells number at adulthood. The indicated strains were crossed with a strain expressing GFP in the seam cells (<i>scm::GFP</i>) and the number of seam cells scored in young adult hermaphrodites grown at 15°C. The mean value and range covered by the individual counts are indicated. <b>C</b>) Representative average of the seam cells at adulthood. The indicated mutants were crossed with a strain expressing GFP in the seam cells (<i>scm::GFP</i>) and scored. The pictures correspond to GFP and DIC fluorescence, the number of seam cell nuclei is indicated within parentheses. The number of animals scored (n) is indicated (<b>A</b>–<b>B</b>).