<i>ceh-14</i> affect differentiation of several neuron classes, in combination with different homeobox genes.

Fig 3A:ceh-14(ot900) mutant animals show a loss of neuropeptide-encoding gene expression in PVW, including a promoter fusion reporter transgene for flp-22 (ynIs50) and a flp-27 CRISPR reporter (syb4413), while expression of the neuropeptide CRISPR reporters for flp-21 (syb3212) and nlp-13 (syb3411) is unaffected. Neuron of interest is outlined in solid white when expressing wildtype reporter colors, and dashed white when one or all colors are lost. Representative images of wild type and mutant worms are shown with 10 μm scale bar. Graphs compare expression in wild type and mutant worms with the number of animals examined listed at the bottom of the bar. P-values were calculated by Fisher’s exact test. Fig 3B: ceh-14(ot900) mutant animals show a loss of AIM marker expression, including an eat-4 CRISPR reporter (syb4257) and NeuroPAL (otIs669) in AIM. Additionally, unc-86(ot1158) as well as mls-2(cc615) mutant animals show expression defects of NeuroPAL (otIs669) in AIM. Neuron of interest is outlined in solid white when expressing wildtype reporter colors, and dashed white when one or all colors are lost. Representative images of wild type and mutant worms are shown with 10 μm scale bars. Graphs compare expression in wild type and mutant worms with the number of animals examined listed at the bottom of the bar. P-values were calculated by Fisher’s exact test. Fig 3C: ceh-14(ot900) and unc-86(ot1158) mutant animals show a loss of neuropeptide-encoding gene expression in AIM using the CRISPR/Cas9-engineered reporter alleles nlp-51(syb2805) and nlp-73(syb4406). mls-2(cc615) mutant animals diminish, but do not extinguish expression in of nlp-51(syb2805) and nlp-73(syb4406) in AIM. Expression in RIP is unaffected by ceh-14(ot900), unc-86(ot1158), and mls-2(cc615). Neurons are outlined in solid black and a dashed black line represents loss of expression. Asterisks indicate ectopic expression of unidentified neurons. Graphs compare expression in wild type and mutant worms (on vs off or on vs dim) with the number of animals examined listed at the bottom of the bar. P-values were calculated by Fisher’s exact test.