Differential effects of <i>let-7</i> target candidates on vulva formation.

<p>(A) Micrographs of the protruding multiple vulva (pmuv) phenotype in <i>lin-28(n719)</i> and the suppression to a single protruding vulva (pvul) when combined with <i>let-7(mn112)</i>. White arrowheads point to protruding vulvas in the mutants. (B) To screen for changes in the pmuv phenotype, 50–100 <i>lin-28(n719)</i> worms were grown to adulthood on vector control or gene specific RNAi plates (x-axis) and scored for percentage of pmuv (y-axis). The bar graphs represent the average percent of pmuv worms as determined from 5 independent experiments. Error bars represent the standard deviation from the mean and the * points to clones that resulted in significant enhancement or suppression in the % of pmuv worms when compared to the control (empty vector), *P<0.05. (C) To screen for changes in the pvul phenotype, 50–100 <i>lin-28(n719);let-7(mn112)</i> worms were grown to adulthood on vector control or gene specific RNAi plates (x-axis) and scored for percentage of pvul (y-axis). The bar graphs represent the average percent of pvul worms as determined from 4 independent experiments. Error bars represent the standard deviation from the mean and the * points to clones that resulted in significant suppression in the % of pvul worms when compared to the control (empty vector), *P<0.05.