FV-induced splenomegaly in mice immunized with adenoviral vectors.

<p>CB6F1 mice were immunized twice with Ad.pIXgp70 in combination with adenoviral vectors encoding specific interleukins. Mice were immunized first with Ad5 vectors and after 4 weeks, mice were boost-immunized with Ad5F35 vectors. Mice of the group Ad.pIXgp70 received in addition a GFP-encoding or empty adenoviral vector so that the total amount of adenoviral particles in all vaccinated groups was the same. Three weeks after the second immunization, mice were challenged with a high dose of FV and spleens were removed and weighed 3 weeks after challenge infection. Statistically significant differences (<i>P</i> < 0.05; Kruskal-Wallis one-way analysis of variance on ranks with Dunns multiple comparison procedure) compared to unvaccinated control mice (#) are indicated. Each dot shows an individual mouse, the horizontal lines indicate mean values. Data are results of two independent experiments with similar outcome.