Increased splenomegaly in co-infected mice is associated with higher viral loads.

<p>A) Experimental Setup: C57BL/6 mice were infected for 14 days with <i>L</i>. <i>sigmodontis</i> (<i>L</i>.<i>s</i>.) and superinfected with FV for additional 20 days. Control groups were either left uninfected or infected with <i>L</i>. <i>sigmodontis</i> or FV only. Spleen weights (B) and number of spleen cells (C) at day 20 p.i. (D) Viral loads were determined in spleen, BM, and LN cells. Each data point represents an individual mouse. Data are combined from 2–4 experiments (n = 2–5 mice per group and experiment). The line shows the mean and statistical significances are indicated between the groups (*p ≤ 0.05, **p ≤ 0.01).