Role of Hazy in the adult eyelet.

<p>(A, B) Wild-type and <i>hazy^−/−</i> mutant eyelets were stained against Sens (red), Rh6 (green) and Elav (blue); z-projection of confocal sections. In <i>hazy^−/−</i> mutant, the eyelet consisted of 12 cells and all of them expressed Sens, while Rh6 expression was restricted to four cells. (C, D) <i>rh6</i>-GFP and <i>rh6^ΔRCSI</i>-GFP eyelets, stained with anti-Rh6 (red) and anti-GFP (green); z-projection of confocal sections. GFP expression was still observed in the eyelets of <i>rh6^ΔRCSI</i>-GFP. (E) UAS-<i>hazy</i> was expressed under the control of <i>chp^4.5</i>-Gal4 in a <i>hazy^−/−</i> null background (<i>hazy^−/−; chp^4.5</i>-Gal4/UAS-<i>hazy</i>) and the adult eyelet was analyzed for Elav (red) and Rh6 (green); z-projection of confocal sections. Normal number of Rh6 expressing PRs was found in <i>hazy^−/−; chp^4.5</i>-Gal4/UAS-<i>hazy</i> animals. (F) Hazy expression was assessed in <i>sens^RNAi</i> when <i>p35</i> was ectopically expressed in the eyelet to keep the cells alive (UAS-<i>sens^RNAi</i>/<i>lGMR</i>-Gal4; UAS-<i>p35</i>/UAS-<i>Dcr2</i>) and stained with anti-Hazy (red), anti-Chp (green) and anti-Elav (blue). Eyelet consists of 12 cells and Hazy was expressed in all the cells in UAS-<i>sens^RNAi</i>/<i>lGMR</i>-Gal4;UAS-<i>p35</i>/UAS-<i>Dcr2</i> animals; z-projection of confocal sections. (G) Hazy expression (red) when a dominant-negative form of EcR was ectopically expressed in Rh5-PRs (<i>rh5</i>-Gal4/UAS-<i>EcR^DN</i>) in the eyelet and stained with anti-Chp (green) and anti-Elav (blue); z-projection of confocal sections. Eyelet consists of four cells and Hazy was expressed in all PR cells. (H) A Model describing the role of Hazy in the larval eye and the adult eyelet.