Effects of Photoshop Mutations on Tracheal System, Nervous System, and Eye Development

<div><p>(A and B) Fluorescence (A) and brightfield (B) images of tracheal dorsal branch terminal cells in <i>y w Upf2^14J FRT^19A/y w FRT^19A, FLP122; btl-GAL4, UAS-GFP/+</i> mosaic L3 larva. Homozygous <i>Upf2^14J</i> clone (arrowhead) expresses GFP at a higher level than contralateral heterozygous control cell (arrow), but clone has formed normal cytoplasmic branches (A) and a normal air-filled lumen in each branch (B). Bar in (A) (for [A] and [B]), 25 μm.</p><p>(C and D) Wild-type (WT) control clone (C) and homozygous <i>Upf1^26A</i> clone (D) in type IV da neurons labeled with <i>ppk-GAL4, UAS-CD8:GFP</i>. Both control and <i>Upf1^26A</i> clones show complex dendritic arborization fields typical of type IV da neurons, although in the latter the aborizations are easier to visualize because of increased GFP expression. Bar in (C) (for [C] and [D]), 100 μm.</p><p>(E and F) Control (<i>y w</i>) wild-type clones (E) and <i>Upf1^13D</i> clones marked with <i>w⁻</i> in adult eyes. Wild-type clones (E) are easily detected as <i>w⁻</i> patches (white areas, demarked with dotted lines) in the <i>w⁺</i> (red) heterozygous eye, whereas only small <i>w</i>⁻ clones or single <i>w</i>⁻ cells are detected in the <i>Upf1^13D</i> heterozygous eye (F). Similar results were obtained with <i>Upf2^14J</i> (not shown).</p><p>(G and H) Similar experiment with <i>Upf2^14J</i> mutant except eye cells not part of the clone were eliminated by GMR-<i>hid</i> technique. <i>Upf2⁺</i> control clones proliferate (G) to form an eye (slight eye roughness is common with GMR-<i>hid</i> technique), as do <i>Upf2^14J</i> clones (H), although the latter are somewhat smaller and rougher than the controls. Similar results were obtained with <i>Upf1^26A</i> and <i>Upf1^13D</i> (not shown).</p>