<i>unk</i> does not regulate cell growth.

<p>(A–C) Phosphohistone H3 expression (PH3, green) posterior to the MF (arrowheads) is similar in eye discs from control, homozygous <i>unk^ex24</i>, or transheterozygous <i>unk^ex24/unk^Df</i> larvae. (D–F) Examples of control, <i>unk^ex24</i> or <i>Tsc1^Q600X</i> clones in the eye disc. Homozygous mutant cells are marked by the loss of GFP (green) and the adjacent twin spot (ts) has stronger GFP expression than the surrounding heterozygous tissue. (G) Quantification of mutant clone versus twin spot size (n = 7 clones for each genotype). (H) Quantification of photoreceptor cell area (control n = 11, <i>Tsc1^Q600X</i> n = 17, <i>unk^ex24</i> n = 15, <i>hdc⁴³</i> n = 27, <i>unk, hdc</i> overexpression (o/e) n = 17). (I–K) Representative examples of individual cells within control, <i>unk^ex24</i> or <i>Tsc1^Q600X</i> MARCM clones in the eye disc expressing membrane-tagged GFP (green) and stained for Bar expression (red). Note the larger size of <i>Tsc1</i> mutant cells in (K). Scale bar: 2 µm. (L–N) Phospho-AKT expression (red in (L),(M),(N) and white in (L′),(M′),(N′)) is not changed in control (L, L′) or <i>unk^ex24</i> mutant clones (M, M′), but is decreased in <i>Tsc1^Q600X</i> mutant clones (N, N′) in the eye disc. Clones marked by loss of GFP expression (green). Anterior is to the left. Data are represented as mean +/− SEM, ***p≤0.001.