Sens is required for Rh5-PR identity and acts in parallel with Otd.

<p>(A, B) Rh5 and Rh6 expression in wild-type and <i>sens^E2</i> mutant PRs during embryonic stage 17, stained with anti-Rh6 (green), anti-Rh5 (blue) and anti-Elav (red); z-projection of confocal sections. No Rh5 expression was seen in <i>sens^E2</i> mutants and all the cells were marked by Rh6. (C, D) Otd expression in wild-type and <i>sens^E2</i> mutant PRs during embryonic stage 15 stained with anti-Kr (green) and anti-Otd (red). Both in wild-type and <i>sens^E2</i> mutants, all the PR nuclei expresses Otd, showing that Otd was not affected in <i>sens^E2</i> mutant; z-projection of confocal sections. (E, F, G) Sens expression (red) in <i>otd^uvi</i>, <i>sal¹⁶</i> and <i>svp^E22</i> mutant PRs during embryonic stage 12. Staining against FasII or Hazy (green), shows that Sens expression was not affected in these mutants; z-projection of confocal sections.