Rescue of the <i>hazy^−/−</i> mutant phenotype in the larval eye.

<p>(A) Heat shock mediated rescue of <i>hazy^−/−</i> mutant at different stages during development and consequence for Rhodopsin expression. White bar indicates no Rhodopsin expression, while black bar indicates Rhodopsin expression. Red arrowhead marks the stage at which heat shock was given. (B–G) All panels show larval eyes stained with anti-Elav (red), anti-Rh6 (green) and anti-Rh5 (blue); z-projection of confocal sections. (B, C) Heat shocks were performed three times at embryonic stage 12. At the L1 stage, Rh5 and Rh6 expression was detected (B), while at L2 stage, Rh5 and Rh6 expression was lost (C). Heat shocks performed at stage 13 (D), stage 15 (E) and at L1 stage (F) did not result in a rescue of Rh5 and Rh6 expression in the larval eyes at L1 in (D, E) and L2 in (F). Heat shocks at embryonic stage 12 and at L2 stage restores Rh5 and Rh6 expression in L3 larvae (G).