Location of LmHP1-GFP in nuclei of silenced-<i>LmDIM5</i> transformants and in the wild type isolate v23.1.3.

<p>The pBHt2-<i>LmHP1</i>-GFP plasmid was introduced in v23.1.3 and in two different silenced-<i>LmDIM5</i> transformants (.1 and .4, see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004227#pgen-1004227-g003" target="_blank">Figure 3</a>). Confocal laser scanning of fresh mycelium of v23.1.3 and of the two different silenced-<i>LmDIM5</i> transformants were observed. DNA was stained by DAPI, revealing the nucleus. One hundred nuclei were observed for the wild type strain or for the silenced-<i>LmDIM5</i>.1 and .4 transformants. Images are representative of all observations. Scale bars represent 5 µm.