Localization of Rpn5-GFP in proteasome mutants defective in 26S assembly/stability.

<p>(<b>A</b>) Wild type, <i>rpn11-m1</i> and <i>rpn11-m5</i> cells expressing Rpn5-GFP were grown in YPDA medium at 26°C during 8 days. For each time point (day), the OD_{600 nm} was monitored, the survival rate performed (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070357#pone.0070357.s001" target="_blank">Figure S1</a>) and the localization of Rpn5-GFP fluorescence was scored as nuclear (blue bar), at the nuclear periphery (red bar) or as cytosolic dots (green bar; n>100 cells for each time point; two independent experiments; error bars report the differences between the two experiments). (*) indicate that the differences in the distribution of the Rpn5-GFP signal in the mutant cells are significant relative to the wild-type cells after statistical analyses (Pearson’s chi-squared test, P values <0.05). (<b>B</b>) Wild type and <i>rpn11-m5</i> cells producing Rpn5-GFP were grown in YPDA medium at 26°C during 7 days. Localization of Rpn5-GFP was scored as in (A) but every day from day 1 to day 5 and at day 7. (<b>C</b>) Comparison of Rpn5-GFP-cytosolic foci apparition between the wild type (grey) and the <i>rpn11-m5</i> mutant (black) for each day. Error bars represent the difference observed between the two experiments and (*) indicates that the difference between the two strains is significant (Fisher’s exact test, P values <0.05).