<p>Wild type, <i>Δump1</i>, <i>Δrpn10</i> and <i>Δspg5</i> cells expressing Rpn5-GFP were grown in glucose containing rich medium (YPD) at 26°C and examined by fluorescence microscopy in the exponential growth phase (EP) and after 5 days in stationary phase (SP). Typical images of Rpn5-GFP localization are shown.</p
<p>Fluorescence imaging was used to evaluate cellular localization of wt GFP-IRF5 or point mutants i...
<p>Cells expressing Xrn1-GFP (strain CRY1703) were observed 3 (A; log phase), 24 (B; diauxic shift),...
(A) Fluorescence microscopy showing localization of CCP5A after fusion of the protein with GFP. CCP5...
<p>Wild type, <i>rpn11-m1</i> and <i>rpn11-m5</i> cells expressing Rpn5-GFP (W303), Rpn1-GFP (W303) ...
<p>(<b>A</b>) Wild type, <i>rpn11-m1</i> and <i>rpn11-m5</i> cells expressing Rpn5-GFP were grown in...
<p>(<b>A</b>) Alignment of the carboxyl amino acid sequences of Rpn11, Rpn11-m1 and Rpn11-m5. The am...
Cells coexpressing the indicated fusion proteins were imaged after 4 d of growth in YPDA at 30°C. GF...
(A) Localization of putative GTP- or GDP-locked mutant of Ypy7p in living cells. Cells were grown to...
<p>(A) In wild-type cells, GFP-Ecm33 clearly localized at the growing cell tips of the cell surface ...
Location of Nsa1-GFP was also determined in and cells that were grown at the permissive temperatur...
(A-D) Analysis of the AP-3 pathway in wild-type and mutant cells. Cells expressing GFP–Nyv1–Snc1-TMD...
<p>Fluorescence microscopy was carried out as described in the Methods section. Cells were grown in ...
(A) Fluorescence microscopy images of exponentially growing Rm2011 rgsP-egfp (gene fusion at the nat...
(A) Fluorescence microscopy images of exponentially growing Rm2011 mVenus-rgsM rgsP-mCherry (both ge...
<p>(A) The Apm1-deletion (Δ<i>apm1</i>), <i>chc1-1</i> mutant and <i>ypt3-i5</i> mutant expressing c...
<p>Fluorescence imaging was used to evaluate cellular localization of wt GFP-IRF5 or point mutants i...
<p>Cells expressing Xrn1-GFP (strain CRY1703) were observed 3 (A; log phase), 24 (B; diauxic shift),...
(A) Fluorescence microscopy showing localization of CCP5A after fusion of the protein with GFP. CCP5...
<p>Wild type, <i>rpn11-m1</i> and <i>rpn11-m5</i> cells expressing Rpn5-GFP (W303), Rpn1-GFP (W303) ...
<p>(<b>A</b>) Wild type, <i>rpn11-m1</i> and <i>rpn11-m5</i> cells expressing Rpn5-GFP were grown in...
<p>(<b>A</b>) Alignment of the carboxyl amino acid sequences of Rpn11, Rpn11-m1 and Rpn11-m5. The am...
Cells coexpressing the indicated fusion proteins were imaged after 4 d of growth in YPDA at 30°C. GF...
(A) Localization of putative GTP- or GDP-locked mutant of Ypy7p in living cells. Cells were grown to...
<p>(A) In wild-type cells, GFP-Ecm33 clearly localized at the growing cell tips of the cell surface ...
Location of Nsa1-GFP was also determined in and cells that were grown at the permissive temperatur...
(A-D) Analysis of the AP-3 pathway in wild-type and mutant cells. Cells expressing GFP–Nyv1–Snc1-TMD...
<p>Fluorescence microscopy was carried out as described in the Methods section. Cells were grown in ...
(A) Fluorescence microscopy images of exponentially growing Rm2011 rgsP-egfp (gene fusion at the nat...
(A) Fluorescence microscopy images of exponentially growing Rm2011 mVenus-rgsM rgsP-mCherry (both ge...
<p>(A) The Apm1-deletion (Δ<i>apm1</i>), <i>chc1-1</i> mutant and <i>ypt3-i5</i> mutant expressing c...
<p>Fluorescence imaging was used to evaluate cellular localization of wt GFP-IRF5 or point mutants i...
<p>Cells expressing Xrn1-GFP (strain CRY1703) were observed 3 (A; log phase), 24 (B; diauxic shift),...
(A) Fluorescence microscopy showing localization of CCP5A after fusion of the protein with GFP. CCP5...