Nuclear localization of transiently expressed wild type and A2^R254A in interphase HeLa cells.

<p>HeLa cells were transfected with GFP vector (first row) wild-type A2-GFP (second row) or A2^R254A-GFP. For the control vector, GFP is diffusely localized throughout out the nucleus and extranuclear region. Both A2-GFP and the A2^R254A-GFP point mutant, which lacks the sole methylated arginine residue, are exclusively observed in the nucleus. The signal from Hoechst dye (blue) is overlaid on both the GFP signal (centre panels) and images obtained by digital interference contrast (DIC) microscopy (right panels) to highlight the location of the nucleus. Scale bar = 10 µm.