GFP-U_S9 localization.

<p>Cells shown here are representative of several experiments and all share the typical GFP-U_S9 pattern, i.e. a bright punctuate signal present throughout the cytoplasm and partially concentrated in a peri-nuclear region. Additionally, GFP-U_S9 can be seen at the plasma membrane, while no fluorescent signal is present in the nuclear region. In the panel labeled MDA hm (higher magnification), the microphotograph focuses on the extension tip protruding from one transfected cell and reaching the body of one untransfected cell. GFP-U_S9 can be detected in vesicles present in this distal region as well as on the plasma membrane. In green: GFP-U_S9 fluorescence; nuclei are stained in blue with Hoechst 33342. Bar = 10 µm.