M71::GFP does not traffic to the plasma membrane.

<p>(A) M71::GFP expression in OP6 cells in trapped perinuclearly (A’) Same cell with colabelling of plasma membrane revealed by CellMask Red. (B) mβ2AR::GFP expression in OP6 cells gives a flat morphology that has sharp focus. Expression is observed in filopodia. (B’) Same cell as (B) with colabelling of plasma membrane revealed by CellMask Red. (C, D) M71::GFP and mβ2AR::GFP expression vectors. (E) Filopodia counts by Cell Mask Red and GFP for mβ2AR::GFP and mβ2AR::GFP. (F) M71::GFP co-expression with mβ2AR::mCherry in OP6 cells maintains GFP fluorescence trapped perinuclearly whereas (F’) mβ2AR::mCherry expression is found in the filopodia.