M71::GFP directed mutations that do not traffic to plasma membrane.

<p>(A) M71::GFP mutations F12D;I13H;L14D;G15V;G16T (FILGG to DHDGG), Y35A, P58V;M59T;Y60N (PMY to VTN), M98E, C169A, Y176T;F177C (FY to TC), C178A and Y217A. None of these mutations gave raise to any GFP-labeled filopodia. (B) M71::GFP mutations with N-linked glycosylation modifications: 4x NQS, 4x NSS, NQS to NGT, NQS to NAT, Nt to K4 Nt. (C) Addition of leader sequences (Kirrel2, 5HT3, Calcumenin, Rhodopsin, Endothelin, LUCY and LUCY-FLAG) to M71::GFP did not relocate GFP expression into the filopodia. (A-C) Co-expression with RTP1S for all M71::GFP mutations did not increase the presence of any GFP-labeled filopodia.