Nuclear localization of NV-GFP protein in RTG-2 cells.

<p><i>A</i>, Schematic diagram of the GFP fusion proteins used to transfect RTG-2 cells. <i>B</i>, RTG-2 cells were transiently transfected with pEGFP-N1, pEGFP/NV(1–111), pEGFP/NV(³²EGDL³⁵), or pEGFP/NV(ΔEGDL). After incubation for 48 h, fluorescent signals were examined using confocal microscopy. Each image is representative of the majority of the cells observed in several fields.