Confocal laser microscopy of U-2-OS cells expressing localized fluorescent proteins.

<p>Confocal laser microscopy of fixed U-2-OS cells transiently expressing fluorescent proteins localized to major cellular compartments. Shown are representative images of eYFP or eGFP detected 48 hours after transfection: (<b>A1-3</b>) pFAST6-eYFP::NLS, (<b>B1-3</b>) pFAST5-eGFP::PTS1, (<b>C1-3</b>) pFAST37-eGFP::c-Ha-Ras, (<b>D</b>) pFAST57-CRT::eGFP::KDEL, (<b>E</b>) pFAST58-COXVIII::eGFP, (<b>F</b>) pFAST59-GalNAcT1::eGFP, (<b>G</b>) pFAST61-b1,4GT::eGFP, (<b>H</b>) pFAST56-a-2,6ST-eGFP. (<b>A1</b>–<b>H1</b>) microscopy with fluorescence filters. (<b>A2</b>–<b>H2</b>) nuclei stained with DAPI (dark blue). (<b>A3</b>–<b>H3</b>) merged pictures.