Chemical transfection confocal microscopy images.

<p>Left: Cy3 and Cy5 tagged DNA duplex (<b>S1:S2</b>) added to cells via chemical transfection using Lipofectamine and imaged using confocal microscopy. Images A/E represents the Cy3 channel; B/F the nuclear stain channel; C/G the Cy5 channel and D/H an overlay of all the channels. Images A–D are excited with a 543 nm laser only. Images E–H are excited with both the 543 and 633 nm lasers. Right: Intracellular fluorescence intensity from images A/C and E/G. Data are expressed as mean ± s.e.m from at least ten cells (p<0.001).