Fluorescent microscopy of EYFP/ECFP fusion proteins expressed in HeLa cells.

<p>EYFP (<i>green pseudocolor</i>) and ECFP-tagged (<i>red pseudocolor</i>) proteins were analyzed in HeLa cells for subcellular localization and co-localization (the “<i>merge</i>” row resulted in a <i>yellow color</i> when colocalization occurred) by fluorescence confocal microscopy. In addition, a line profile plot (<i>right</i>) shows the pixel intensities of EYFP and ECFP along an arrow (distance in µm) presented in the “merge” figure. <i>A,</i> NFS1-EYFP and ISD11-ECFP (mitochondrial localization); <i>B</i>, NFS1-EYFP (cytosolic localization) and ISD11-ECFP (nuclear localization); <i>C</i>, EYFP-NFS1Δ1-55 and ISD11-ECFP; <i>D</i>, <i>E</i> NFS1-EYFP and ECFP-MOCS3; <i>F</i>, EYFP-NFS1Δ1-55 and ECFP-MOCS3. The figures of <i>panel E</i> are a <i>close-up</i> figure of an indicated area (box) in the figures of <i>panel D</i>. Scale bars, 20 µm (except <i>E,</i> which is 2 µm).