Subcellular localization of OatA^TM10-YFP and OatB^TM10-YFP fusions.

<p>A) Schematic representation of fusion proteins (YFP, yellow star) and corresponding micrographs in phase contrast (PC) microscopy and fluorescence microscopy (YFP). Upper panels, <i>oatA</i> mutant producing cytoplasmic YFP (OatA^−/YFP) used as control; middle panels, <i>oatA</i> mutant producing OatA^TM1–10-YFP (OatA^−/OatA^TM1–10-YFP); lower panels, <i>oatB</i> mutant producing OatB^TM1–10-YFP (OatB^−/OatB^TM1–10-YFP). Induction of expression was performed with 10 ng/ml of nisin. Bar scale, 2.0 µm. B) Fluorescence ratio (FR; AU, arbitrary unit) between the fluorescence measured at mid-cell position and pole. A^−/A™, <i>oatA</i> mutant producing OatA^TM1–10-YFP and B^−/B™, <i>oatB</i> mutant producing OatB^TM1–10-YFP. Lines represent the mean value (n = 20, 3 independent replicates).