Effects of mutating Leu64 and Lys65 residues on the subcellular distribution of α_2A-AR.

<p>GFP-tagged wild-type (WT) α_2A-AR and its mutants L64A, K65A and LK-AA were transiently expressed in HEK293 (<i>upper panel</i>) and HeLa cells (<i>lower panel</i>) and their subcellular distribution of the receptors was revealed by detecting GFP fluorescence by confocal microscopy. The data shown are representative images of at least three independent experiments. <i>Green</i>, GFP-tagged receptors; <i>blue</i>, DNA staining by DAPI (nuclei). Scale bar, 10 µm.