Localization of the GMPs in interphase nuclei.

<p>Schematic illustration of the domain organization of the GMPs included in the study. For nomenclature and references see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062783#pone-0062783-t001" target="_blank">Table 1</a>. (a) Protein domains were identified as regions of high homology using Clustal W multiple sequence alignments. The C-terminal episome-binding domains are indicated in yellow and the chromatin targeting modules in blue. The Gly-Ala or Gly-Ala-Ser repeats of LCV-GMPs and the acidic repeats of RHV-GMPs are indicated in green and red, respectively. The acidic tails of LCV-GMPs are shown in pink. (b) Expression of GFP tagged GMPs in transfected U2OS cells. Representative western blot of total lysates from U2OS cells harvested 48 hrs after transfection probed with an anti-GFP antibody. (c) Nuclear localization of the transfected proteins. Representative fluorescence micrographs of NIH3T3 cells transfected with the indicated GFP-GMP fusion proteins or control GFP-NLS. DNA is visualized by DAPI staining (blue). The merged image and localization profile indicate that the GMPs do not accumulate on heterochromatin.