Dms1 localizes at the SPB and the nuclear envelope.

<p>(A) Localization of GFP-Dms1 during vegetative growth and sporulation. A homothallic haploid wild-type strain expressing GFP-Dms1 (CAL81) was grown on YEA for 1 day or was sporulated on MEA for 16 hours. Chromosomal DNA was stained by Hoechst 33342 and analyzed by fluorescence microscopy. GFP-Dms1 (green) and Hoechst 33342 (blue) are overlaid in the merged images. Bar, 10 μm. (B) Dual observation of Dms1 and microtubules, the nuclear envelope, or meiotic SPB components. Homothallic haploid wild-type cells expressing GFP-Dms1 and Cut11-3mRFP (CAL98), mCherry-Atb2 (CAL84), Spo13-mCherry (CAL88), or Spo15-mCherry (CAL119) were sporulated on MEA for 16 hours. GFP-Dms1 (green), Cut11-3mRFP, mCherry-Atb2, Spo13-mCherry, or Spo15-mCherry (magenta) and Hoechst 33342 (blue) are overlaid in the merged images. Bar, 10 μm. High magnification-images of the regions in the white squares are shown below. Bar, 1 μm.