Conditional expression of GlDRP-K43E elicits a mitosome morphogenesis phenotype.

<p>(A-C) Subcellular localization of a C-myc tagged <i>Gl</i>Tom40 (red) by IFA in cells induced to express a wild type <i>Gl</i>DRP (green) or GTP-locked variant <i>Gl</i>DRP-K43E (D-F). Note the altered size and distribution of organelles labeled with Tom40-myc in <i>Gl</i>DRP-K43E expressing lines. Nuclear DNA is stained with DAPI (blue). Insets: DIC images. Scale bar: 1 μM (G) -Cell fractionation experiments confirm fixed membrane localization of <i>Gl</i>DRP-K43E. (H) TEM: normal morphology of mitosomes (black arrows) in the CMC in cells expressing wild type <i>Gl</i>DRP whilst cells expressing <i>Gl</i>DRP-K43E show enlarged dumbbell-shaped mitosomes (black arrows in I, J) indicative of defective organelle division. Nu: nucleus. Scale bars: 100 nm.