Validation of comparative RNA-Seq analysis.

<p>(A) qRT-pCR analysis of representative genes identified by RNA-Seq. Values represent the average transcript copy numbers for each gene normalized per <i>lipL32</i> transcript. Bars indicate the standard error of the mean (SEM). <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004004#s2" target="_blank">Results</a> presented are mean values from at least 3 biologically-independent samples of leptospires for each growth condition. The fold-regulation for each gene determined by RNA-Seq is indicated in parentheses. The fold-regulation between <i>in vitro</i>- (IV) and DMC-cultivated leptospires determined by qRT-PCR are indicated. P values were calculated using an unpaired <i>t</i>-test. (B) Correlation coefficient (R²) between RNA-Seq and qRT-PCR data.