The expression of microRNAs in transduced DU145 cells.

<p>DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. These treatments were followed by isolation of mRNA and then real-time RT-PCR analysis of selected microRNA. All data were normalized against levels U6 expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P<0.05 versus Ad-CMV-EGFP–transduced. A. Up and down-regulated microRNAs were screened by Real-time PCR Array in DU145 cells. B. Validation of the microRNA expression using Real-time PCR.