Validation of miRNA expression by quantitative Real-Time PCR.

<p>Total RNA out of the different samples used for sequencing was reverse transcribed and the expression of miRNAs was analysed in a light cycler by quantitative Real-Time PCR. The relative quantification was carried out by the 2^−ΔΔct-method after measurement the amount of 5.8sRNA in each sample. Represented are the comparisons between (A) EBV-positive lymphomas and thymus, (B) EBV-negative lymphomas and thymus as well as (C) EBV-associated and EBV-negative lymphomas. The shaded bars represent the sequencing results and the filled bars the PCR-results. Bars above the x-axis show induced miRNAs and the bars below repressed miRNAs.