Northern blot and stem-loop real-time PCR analysis of differentially expressed miRNAs before and after transdifferentiation of AR42J-B13 cells.

<p>The differentially expressed miRNAs identified by miRNA microarray were confirmed by miRNA Northern blot (A) and stem-loop real-time PCR analysis (B and C). Total RNA extracted from Dex/OSM treated AR42J-B13 cells (7 Days) and mock controls were used for Northern blot analysis using antisense probes against down-regulated miRNAs (miR-93, miR-106b and miR-130b) and up-regulated miRNAs (miR-21, miR-22, miR-122a and miR-182). The relative fold changes of stem–loop real-time PCR were normalized to AR42J-B13 cells before transdifferentiation and U6 was used as a loading control. (*** P<0.001).