The miRNA expression profile of transdifferentiated AR42J-B13 cells is most closely related to those of hepatoma cell lines by clustering analysis.

<p>The expression levels of miRNAs of various hepatoma cell lines (HepG2, Huh7 and Q7 cells), primary tissues (rat spleen, pancreas and liver), and NIH3T3 cells, were measured by stem-loop real-time PCR analysis. Clustering analysis using Genespring V11.0 software was performed by the normalization of the expression level from each sample relative to that of AR42J-B13 cells. Both up-regulated miRNAs (miR-21, miR-22, miR-122a and miR-182) and down-regulated miRNAs (miR-17-5p, miR-18a, miR-93, miR-106a, miR-106b, miR-130b and miR-375) were chosen as a parameter for comparison.